Regulatory

Part:BBa_K3093003:Design

Designed by: Miao Zongjie   Group: iGEM19_ECUST_China   (2019-10-19)


chbB promoter with a new -35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA"

Source

Escherichia coli str. K-12 substr.MG1655.

References

[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.